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MeltonVEC™-iPSC Reprogramming Kit （Sendai virus)
MeltonVEC™-Cell Fusion Kit (HVJ-E)
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Home > All Collections > Life Science > MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)
  • MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)
  • MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)
  • MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)
  • MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)

MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)

No.MSV-1002
MeltonVEC™-Cell Fusion Kit is a highly effective cell fusion reagent product that uses the Sendai virus envelope (Hemagglutinating virus of Japan Envelope; HVJ-E) to inactivate the Sendai virus RNA genome, resulting in non-proliferative and non-infectious vesicle HVJ-E. It can be used in biosafety level 1 (BSL1) laboratories.Cell fusion efficiency exceeds 90%.
$820.00
kit:
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  • MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)
  • MeltonVEC™-Cell Fusion Kit (HVJ-E)（Liquid)

SPECIFICATION


(Technical principle)






Nuclear-transfer Test in International Center for Primate Brain Research(ICPBR) ,Shanghai city, China


                                                                                   


Product Application Areas:

  • The preparation of hybridoma-HVJ-E cell fusion reagent promotes cell membrane fusion by binding its envelope protein with the host cell membrane, resulting in the formation of hybridoma cells with the ability to produce monoclonal antibodies and unlimited proliferation potential.

  • Spindle Transfer: These reagents facilitate spindle transfer procedures, a technique commonly used in reproductive biology to transfer the spindle apparatus between oocytes, supporting successful cell fusion and genetic manipulation. (The recommended soaking time in spindle transplantation experiments should not exceed 5 seconds.)

  • VLP（Gene Delivery Vector): Plasmids, decoy nucleic acids (DNA), and small interfering RNA (siRNA), as well as messenger RNA (mRNA), are macromolecules that require a delivery system called a "vector" to be efficiently introduced into target tissues or cells. HVJ-envelope (HVJ-E) uses the virus’s membrane fusion mechanism, allowing for the active delivery of DNA or RNA into target cells in a manner similar to injection. Unlike passive uptake via phagocytosis, this method involves the vector itself fusing with the cell membrane, offering a more efficient delivery. While utilizing the virus’s membrane fusion mechanism, HVJ-E does not use the viral genome, making it a non-viral hybrid vector. Initially developed for gene and nucleic acid delivery, it has now progressed to clinical trials as a therapeutic for cancer and infectious diseases, attracting attention as a novel gene nucleic acid delivery vector. 

Product Usage Instructions

1. Upon receiving the product, please immediately check the dry ice inside the insulated container. Proper low-temperature storage is critical to maintaining the product's performance and may affect cell fusion efficiency.(We recommend that liquid nitrogen be used for quick freezing immediately after packaging to maintain activity)

2. Contact time between virus and cells After testing, other customers believe that 30s-45s is more appropriate (monkey cells), but too long contact time between virus and cells may cause cell death. The contact time between virus and cells may need to be optimized to achieve the best experimental results.

3.The cell fusion reagent is used in spindle transplantation experiments, with the spindle being immersed for approximately 5 seconds. If the time is too long, the spindle will die. Note：Experimental tip: A glass syringe can be used to gently maneuver the cells, allowing them to come into contact with more virus particles in the HVJ-E solution.


Product Name Catalog
Number		 Packing volumes Specifications Price Note
MeltonVEC™-Cell Fusion Kit
(Liquid)		 MSV-1002 260ul/tube
(According CoA file)		 1 tube /kit USD 820.0 Transportation and customs clearance fees need to be discussed separately

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