Sendai Virus Vector Engineering Platform

SENDAI VIRUS VECTOR PLATFORM
Efficient, Non-integrating RNA Delivery for Advanced Cell Engineering

Melton’s Sendai Virus Vector Engineering Platform provides an efficient, non-integrating solution for RNA-based gene delivery. It enables rapid and robust expression of target genes in both dividing and non-dividing cells, including primary and difficult-to-transfect cells. With flexible multi-gene and F-deficient vector designs, the platform supports iPSC reprogramming, cell engineering, and customized gene delivery applications.


KEY ADVANTAGES OF SENDAI VIRUS VECTORS

High Transduction Efficiency
            Efficiently delivers target genes into primary and difficult-to-transfect cells.

Non-Integrating Delivery
            The RNA genome remains in the cytoplasm without integrating into the host genome.

Rapid and Robust Expression
            Enables fast and high-level expression of target genes after delivery.


F-DEFICIENT SENDAI VIRUS VECTOR DESIGN
Controlled, Single-Round Gene Delivery with Enhanced Biosafety

The F protein is essential for membrane fusion and the production of infectious progeny. In the F-deficient Sendai virus vector (ΔFSeV), the F gene is deleted from the viral genome and supplied only during vector production. This design enables efficient initial gene delivery while preventing the generation and secondary spread of infectious progeny in target cells.

Schematic design of the F-deficient Sendai virus vector (ΔFSeV). Deletion of the F gene prevents the production of infectious progeny virus particles in target cells.

KEY ADVANTAGES OF F-DEFICIENT SENDAI VIRUS VECTORS

• Enhanced Biosafety

• Expanded Transgene Capacity